ylyB [2017-11-27 18:23:02]

similar to pseudouridylate synthase

locus: BSU15460

pI: 5.43

mw: 33.50 kDa

protein length: 303 aa

gene length: 909 bp

function: RNA modification

product: putative pseudouridylate synthase

essential: no

ec: 5.4.99.23

synonyms: ylmL

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.3|rRNA modification and maturation/ based on similarity]

Gene

Coordinates: 1,617,210 → 1,618,121

The protein

Catalyzed reaction/ biological activity

• RNA uridine = RNA pseudouridine (according to Swiss-Prot)

Protein family

• S4 RNA-binding domain (according to Swiss-Prot)

Paralogous protein(s)

• [protein|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|YjbO], [protein|064AE8CE6D52E35582F90654AA9548963573C05C|YhcT]

Structure

• [PDB|2I82] (the ''E. coli'' enzyme) [Pubmed|17188032]

Expression and Regulation

Operons

• genes: [gene|8FC1A0A6C9975F6C125490FBAAB2A4957210A9F1|lspA]-[gene|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|ylyB]-[gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE15460 (Δ[gene|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|ylyB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGCGGTTATATCAATTTGAT, downstream forward: _UP4_TGACAGAGGGTTTCTTTTCT
• BKK15460 (Δ[gene|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|ylyB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGCGGTTATATCAATTTGAT, downstream forward: _UP4_TGACAGAGGGTTTCTTTTCT

References

• 17188032